Place it immediately after the on-screen hit, then follow with the Bruh sound for a deadpan reaction or the Emotional Damage sound when the loss needs a bigger payoff, keeping that moment as the setup for the joke that follows.
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This short clip captures Mario's hurt cry as he takes damage, lasting just over two seconds with his familiar high-pitched voice front and centre. It feels comic and sympathetic at once, like watching a cartoon hero bounce back from a clumsy knock. The sound comes from the Mario games, where that yelp marks the moment he takes a hit.