Place it at the very beginning of an edit, before any gameplay or travel footage, then follow it with Okay Let's Go as a second start cue, or cut to CJ Here We Go Again when the plan turns chaotic, and save Run Vine for the moment the chase actually starts.
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This under-two-second clip is Mario saying his let's-a-go catchphrase in a bright, high-pitched Italian-accented voice, full of bounce and readiness. It feels joyful and instantly energising, like a starting signal that makes listeners smile and lean in for what comes next. The line comes from Nintendo's Super Mario games, where it has been used across many adventures as a cheerful call to begin.