Put it at the peak of a fail sequence, after a short build-up, then follow it with the FNAF Jumpscare Scream for a second hit or the Roblox Oof Sound to close the moment.
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This is Mario's loud, exaggerated scream from the Super Mario games, a high-energy cartoon yell lasting about nine seconds. It feels like sudden, over-the-top panic, funny because the disaster it signals is so dramatic and instantly familiar. The clip comes from Nintendo's long-running Super Mario series and is widely reused as a quick reaction sound for falls, missed jumps and plans falling apart.