Place it at the end of a failed jump or stunt, after Mario Jump sets up the attempt and the fall is already clear. Follow it with Vine Boom for a hard landing, or let it stand alone when the reaction is the whole joke.
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This 16-second clip is Mario letting out a long, drawn-out scream in his high, cartoonish voice, rising and falling as the panic builds. It feels frantic and ridiculous in the best way, like a tiny disaster getting bigger with every second. The over-the-top delivery makes it funny rather than alarming, turning a small mishap into a full cartoon meltdown.