Place this clip first as the immediate reaction, then follow a bigger fail with the Mission Failed Meme Sound for the payoff, or precede a chase sequence with the Running Sound Effect so the bump interrupts the movement.
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This clip is Mario's single low-pitched 'ungh' grunt from Super Mario 64, lasting under half a second and sounding like a small bump or effort. It feels funny and instantly awkward in a cartoonish way, turning a tiny mistake into a reaction without needing any words, which is why listeners recognise it straight away.