Place it at the exact beat of the failed jump, then follow with the Roblox Oof for a second, heavier fail, and finish with the Danger Alarm Meme Sound for the moment the run is clearly over.
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This 0.5-second clip is Mario's brief, strained 'yugh' grunt from Super Mario Sunshine, the sound he makes when a jump goes wrong and he takes a hit. It lands as a small, comical wince rather than a big scream, funny and slightly pained at the same time, the kind of noise that makes a failed jump feel instantly recognisable to anyone who has played the game.