Place this clip at the cut point before a reaction lands, then follow it with the Bruh Soundboard Button for a longer payoff, or precede a chaotic clip with the Scream Meme Sound Button and let this one close the sequence.
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This clip is a single less-than-a-second 'meeep' noise, a short high-pitched squeak stretched into one syllable. It feels silly and startling in a harmless way, like a tiny cartoon horn cutting in out of nowhere, which makes the interruption itself the funny part for listeners expecting a normal reply or sound.