Place it near the end of a short reaction sequence, after Sudden Suspense and before Oh My God Wow, then close with Kids Cheering when the group joins in for the payoff.
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This 1.4-second clip is a wordless tiger roar, delivered as one animal call. It feels bold and comic at once, a quick flash of predator attitude that suits a joke or a playful challenge. It comes from Mellqui Costa's roar during a live stream featuring Diego, the saber-toothed tiger from Ice Age.