Place this clip right after the bad reveal in your edit, preceded by the dun dun dun sound for the build-up and followed by the vine boom sound to close the sequence, keeping other reaction clips for a separate beat later.
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This clip is a single drawn-out human scream lasting just under four seconds, voiced as a continuous AAAAAAAAAH with no words or other sounds behind it. It gives a listener a startled, over-the-top panic feeling, turning a small shock or bad reveal into something funny through sheer volume and exaggeration.