Place it at the turn from sneaking to spotted, then move straight to the chase. Follow it with the Run Vine sound as everyone scatters, or drop in the Bruh sound for a deadpan beat after the cover is blown.
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This is the short, sharp alert sting from Metal Gear Solid, lasting about one and a half seconds, with a sudden rising burst that signals danger. It gives an instant jolt of tension and comic panic, like being caught red-handed. In the games it plays when an enemy spots Snake and the stealth cover is blown.