Place it immediately after the loudest beat in your edit, letting a jumpscare or alarm hit first and this clip answer it. Follow a FNAF jumpscare scream or a danger alarm with it, then cut to the reaction shot so the ear complaint closes the gag before the next scene starts.
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This under-a-second voice clip is a single stretched-out shout of 'mis oídos', Spanish for 'my ears', drawn out across repeated vowels as if the speaker has just been hit by something deafening. It lands as a pained, comic overreaction, the kind of dramatic wince that turns a loud moment into a joke without needing any extra explanation.