Place it immediately after the accusation in your edit, so the denial answers the blame directly. Open with Sudden Suspense before the accusation, then let this clip carry the reply. Follow it with Oh My God Wow for the group's reaction to the denial.
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This is a Portuguese voice clip lasting about fourteen seconds, built around one emphatic denial: the speaker insists they did not kill Ester. The delivery is tense and dramatic, making the denial sound desperate rather than calm. That over-the-top seriousness gives it an awkward, darkly funny edge, which is why it works as a reaction to sudden blame.