Place it immediately after the setup line and before the reaction shot, then follow it with Oh My God Wow to extend the reaction, and close the sequence with Nelson Ha Ha for the closing laugh.
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This is a very short spoken reaction clip, lasting about two seconds, built around a single abrupt vocal burst. It lands with sudden awkward surprise, giving listeners a quick jolt of shock that makes a scene feel stranger, more uncomfortable and funnier at once, without needing any extra context or explanation.