Put it at the end of a short failed-attempt edit, straight after the Inception Horn builds the moment up and just before the Nope Meme delivers the reply, so the reaction that follows it lands as the payoff.
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This clip is a very brief stretch of pure silence, lasting just 216 milliseconds, with no voice, music or sound effect in it at all. It feels awkward and anticlimactic in the best way, like a joke with the punchline removed, leaving listeners waiting for something that never arrives.