Place it right after the bad suggestion appears on screen, then follow it with the Bruh sound for a double rejection, or use Emotional Damage first to build up the reaction before this final no.
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This is a single spoken 'no' stretched by a heavy reverb tail, lasting about three and a half seconds. The echo makes the refusal feel bigger and more dramatic than a flat no, turning a simple answer into a firm shutdown. It feels funny and defiant, like slamming a door on a bad suggestion without raising your voice.