Place it in the middle of a prank edit, after the setup and just before the reveal, then follow it with the Shocked Meme Sound for the reaction shot and the Why Are You Running sound to carry the chase that follows.
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This is a short spoken outburst lasting about twelve seconds, delivered as a startled reaction in the middle of a horror parody scene. It feels sudden, silly and over the top, turning a frightening moment into a laugh. The clip comes from Scary Movie 3, where the joke lands because the panic is played for comedy rather than fear.