Place it as the reaction beat right after the setup line lands, before the payoff shot cuts in. Pair it after a sudden suspense sound to build expectation, then follow it with a damn reaction for the fallout, keeping it as the middle link in the chain.
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A very short voice clip of someone exclaiming 'oh boy' in a flat, resigned tone, lasting just under a second and a half. It gives listeners that sinking, here-we-go feeling of watching a mistake unfold or bracing for awkward news, turning mild dread and reluctant anticipation into a quick, dry punchline.