Put it immediately after the mishap, as the reaction beat before the next joke lands. Let Cartoon Slip set up a fall first, then follow this clip with Wrong Answer Game Show when the mistake is confirmed, so the sequence moves from accident to realization to verdict.
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This is a short 1.6-second voice clip of Ian saying “Oh no!” in a flat, defeated tone, with the two words delivered quickly and clearly. It feels instantly dismayed and slightly resigned, like someone watching a small mistake turn into a bigger problem. That weary, understated delivery makes the reaction funny rather than dramatic, and easy to recognize in an instant.