Place this clip at the cut point after the visual payoff, then follow it with the awkward moment sound if the scene needs a longer tail. For a mistake sequence, put the Windows XP error sound first and let this clip act as the reaction afterwards.
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This is a single voice clip, under a second long, of one person saying just “oh” with nothing else behind it. Because it is so plain and short, it reads as surprise, a sudden realisation, mild disappointment or an awkward beat, with the exact feeling shaped by whatever moment it follows.