Place it as the first reaction after the surprising line in the edit, then follow it with Bruh for a flat second beat and Emotional Damage when the joke needs a bigger finish.
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This is a single voice saying the Portuguese exclamation Orra Diacho, with the phrase stretched across about five seconds. The delivery feels startled and exasperated, with a comic edge that makes disbelief sound larger than life. It leaves the listener with a sense of comic disbelief, as if something absurd has just happened.