Place it at the exact frame of the reveal as the first reaction, then follow it with a huh sound button for a second confused beat, or precede it with sudden suspense to build into the moment before the payoff lands.
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This clip is a single short vocal exclamation stretched into 'Oyaaaaa' over about one and a quarter seconds, with one voice carrying the whole shout. It feels playful and caught off guard, like a cartoonish double take, turning mild surprise into an exaggerated, funny reaction that listeners instantly read as disbelief.