Place it as the payoff after a short setup clip, then follow it with the Awkward Moment sound for a second beat before the WTF Boom sound closes the scene with a bigger impact.
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This five-second clip is a short, irritated anime voice line delivered in Tomura Shigaraki's frustrated tone, with the speaker snapping out the phrase in a sharp, fed-up delivery. It feels annoyed, confrontational and darkly funny, like a villain losing patience mid-conversation. That edge makes it work as an instant reaction to something irritating, rather than a calm remark.