Place it just after the reveal or failed plan, then follow it with No God Please No for a bigger reaction, or cut to What Da Dog Doin when the joke needs a confused follow-up instead of a dramatic one.
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This is a twelve-second recording of Mr. Popo's laugh from DBZ Abridged, a high, wheezy cackle that keeps going far longer than a normal laugh. It feels funny at first, then unsettling and awkward, as if the character knows something you do not. The exaggerated delivery is what makes it stick as a reaction sound for Dragon Ball fans.