Place it at the exact hit point in your edit, after a build-up sound and before the reaction. Follow it with the scream meme sound button for the target's response, then cut back to normal dialogue for contrast.
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This is an extremely short laser sound effect, lasting just a fraction of a second as a sharp sci-fi zap. It feels punchy and playful rather than threatening, giving a tiny blast the comic energy of an overpowered weapon fired for a joke, a surprise hit, or an exaggerated reaction in the moment.