Place it at the end of a failed attempt, after the build-up has peaked, then follow it with Nelson's Ha-Ha for a second hit. For a longer gag, open with Sudden Suspense before the reveal so the laugh lands as the payoff.
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This two-second clip is a single burst of exaggerated pwahaha laughter, delivered in a high, theatrical voice that stretches each syllable. It feels gleefully mocking, like someone revelling in a rival's slip-up, and its cartoonish overstatement makes the joke land harder than a plain chuckle would.