Place it at the exact frame your subject stops reacting, right after the build-up shot. Lead in with the Danger Alarm Meme Sound Button for fake urgency, then let this clip undercut it, and follow with the Roblox Oof Sound Button only if someone actually takes the hit.
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This is Quagsire's cry from the Pokémon games, a sub-second vocal sound from the famously laid-back Water and Ground type. It lands as pure dopey indifference, funny because the creature sounds completely unbothered by whatever is happening around it, which makes even a serious moment feel suddenly silly and slow.