Put it at the end of a joke or fail, after the setup has played out, then follow it with a funny laugh for a bigger reaction, or an evil laugh when the joke is mean-spirited, and finish with a drum sting to close the bit.
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This is a ten-second clip of a frog making a long, croaking sound that rises and falls like a laugh, in the Brazil version of the clip. It feels silly and a little absurd, the kind of unexpected animal noise that makes a plain joke land harder because the laugh comes from a frog instead of a person.