Place it at the peak of a reaction, after the setup and before the payoff, then follow it with a Vine Boom to close the moment, or lead into it with an Anime Wow for a double take. Use it once per edit so the moment keeps its impact.
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This clip is an animal scream lasting about 3.2 seconds, with animal noise and no speech in it. It feels sudden and absurd, like a creature reacting in pure panic, and that over-the-top alarm is what makes it funny to hear.