Put it at the very start of a reveal, before the creature or boss is shown, so the moment builds around it. Follow it with the Inception Horn for extra scale, or cut straight to the FNAF Jumpscare Scream when the threat finally appears on screen.
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This 4.6-second clip is a single deep horn toot, blown as if calling a creature far larger than the person sounding it. It feels heavy, ominous and strangely playful, like a signal meant to be heard across water and mountains. It comes from God of War, where the horn is used to summon the World Serpent.