Place it immediately after a loud opener in your edit, then follow the gap with a new scene sound. Pair it after the vine boom sound button for a hard reset, or before the mlg air horn sound button when you want the next hit to stand alone.
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This clip is just over five seconds of complete silence, intended to stop or override other sounds playing at the same time. It gives a listener an instant feeling of calm and awkward pause, like the room suddenly went quiet after too much noise, which is exactly why the empty moment lands as a joke.