Place it at the end of a reveal, after Sudden Suspense builds the moment, then follow it with Nelson's mocking ha-ha to keep the joke going before cutting to the reaction shot.
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This two-second clip is a piercing, high-pitched laugh in the voice of Skeletor, the skull-faced villain from Masters of the Universe. It builds into fast, delighted cackling that feels smug, theatrically menacing and deliberately silly at once. It is presented as his laugh from the 1983 He-Man animated series, though its exact episode is not confirmed.