Place it at the reveal point in an edit, after a dun dun dun sting has set up the turn, and follow it with a hard cut to your next scene instead of layering more effects on top.
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This is a three-second screech built around a harsh, high-pitched scream-like effect with no clear words. It lands as tense and unsettling rather than funny, the kind of sharp hit that makes a normal smile feel wrong and puts listeners on edge instantly.