Use it as the payoff after a small scheme collapses on screen, then follow it with Mission Failed for a bigger defeat or Ba Dum Tss when the failure is meant to stay light. It also works before Oh Brother This Guy Stinks when someone else reacts to the same mistake.
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This short voice clip catches Plankton letting out a sharp, exasperated augh after something goes wrong, with his small, scheming voice carrying the whole outburst. It feels frustrated and defeated, but in a funny, over-the-top way that makes a setback sound dramatic instead of serious. The line comes from Plankton, the tiny villain from SpongeBob SquarePants.