Place it right after the mouse is spotted in your edit, then follow it with a Vine Boom and a SpongeBob Fail when the chaos settles, keeping the mouse as the centre of the joke.
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This clip is a 12-second mouse scream, a sharp, high-pitched animal cry from a tiny mouse. It feels funny and startling at once, because the sound is far bigger and more dramatic than the small animal making it, turning a simple squeak into an over-the-top reaction.