Place it right at the death frame in your edit, before any commentary or retry footage. For a montage of fails, alternate it with the Roblox Oof sound button on the next death so each attempt gets a different payoff and the sequence does not repeat itself.
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This is a six-second game effect built around Mario losing a life, with the familiar descending death jingle playing out in full. It feels deflating and funny at the same time, turning a small mistake into an instantly readable game over moment for anyone who grew up with platformers.