Place it at the end of a short fail sequence, after the moment of defeat has already landed, then follow it with SpongeBob Disappointed for a second reaction before cutting to the next scene.
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This short clip is the Pokémon Torkoal's cry, a wavering, high-pitched wail that lasts just over two seconds and sounds like a small creature on the verge of tears. It feels pitiful and overdramatic at once, the kind of sad noise that makes a setback seem funny instead of serious.