Place it in the middle of a troll edit, right after the bait is set and just before the victim reacts, then follow it with a shocked meme sound for the payoff and a goofy yell when the prank collapses completely.
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This 9.9-second clip is a drawn-out, exasperated 'ughhhhhh' groan delivered in Plankton's squeaky, scheming voice, stretched out as if the tiny villain is milking every second of his irritation. It lands as pure comic exasperation, the sound of someone fed up with a joke that has gone on far too long.