Put it at the exact frame the failure becomes undeniable, after the setup line and before the reaction shot. For bigger flops, precede it with the Dun Dun Dun Sound Button, or follow a smaller mistake first with the SpongeBob Fail Sound Button.
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This clip is a five-second sad trombone-style trumpet playing the classic descending wah wah wah wahhh line used for a defeat. It turns a small failure into a comic ceremony, making the moment feel deflated, sheepish and instantly readable as a flop everyone saw coming.