Place it right after the flirty line or shy look, as the reaction that closes the beat, then follow it with the Rizz Sound Effect for a confident contrast or the Baby Laughing Meme Sound Effect to keep the joke light. Keep it as a single reaction tag between scenes rather than a running gag.
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This is a very short, high-pitched voice clip repeating the UwU sound in a squeaky, exaggerated delivery. It lasts just over a second, so the cute vocal burst lands instantly and then stops. It feels playful, awkward and deliberately over-sweet, like a joke reaction to something flirty, wholesome or a little too adorable for the moment.