Place this clip at the very end of a reaction sequence, after a setup beat from Sudden Suspense Sound Button, then follow it with Nelson's Ha-Ha! Sound Button if you want a second, meaner laugh to close the edit out completely.
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This is a very short human laugh, just over half a second long, with an odd, strained tone that does not sound like a normal chuckle. It gives listeners an awkward, slightly unsettling feeling, funny because the laugh itself feels off and out of place in the moment.