The line comes from Grand Theft Auto V, Rockstar Games' 2013 open-world game, where Trevor Philips is one of three playable protagonists known for his unpredictable temper. Short voice clips from Trevor became popular on soundboards and in edited gameplay videos because his delivery is instantly recognisable and easy to drop into a reaction. This particular question works as a standalone confrontation, so creators reuse it far beyond the original story, whenever they need a fast, distrustful response to someone appearing unexpectedly or hiding something.