Put it in the middle of a short skit, after the setup shows the request being ignored and before the payoff. Follow it with Why Are You Running when the other person finally gives in and runs off, or cut to Shocked Meme Sound when they refuse one last time.
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This is a short spoken clip built around the phrase 'why won't you', running for about eleven seconds. The delivery sounds pleading and frustrated, like someone asking the same question again because they are not getting the answer they want. It feels exasperated and a little funny, the kind of exasperation that lands when a simple request keeps being ignored.