Place it at the exact frame a popup or message box appears on screen. Follow it with a bell ding for a success beat, or precede it with an error sound when the prank is meant to look like a system failure.
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This is the short Windows notification chime, a clean two-note system alert lasting just over a second. It feels instantly familiar and mildly urgent, like something on your computer needs attention right now. The clip comes from the Windows operating system, where it plays for new alerts and system messages.