Place this clip after the setback itself, once the result is already clear, then follow it with the Sad Violin Sound Button to extend the reaction into the next shot. If the scene needs a lead-in, put Sudden Suspense Sound Button right before the reveal and keep this clip as the payoff.
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This is a just-over-a-minute reaction clip centred on a drawn-out cry of woe, stretched far beyond a quick exclamation. The length lets the dismay build instead of passing in a second, which gives it a heavy, resigned feeling. It lands as gloomy and a little theatrical, the kind of sorrow that feels performed for everyone listening rather than kept private.