Place it right after the moment of mock despair in a short edit, then follow it with Anime Ahh for a second exaggerated reaction, and save Eh Eh Ehhhh for the closing beat when the joke needs one last push.
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This two-second clip is a single drawn-out wuuu vocal, held on one stretched syllable with no other words or music around it. It feels playful and over-the-top, like a mock wail used to sell a dramatic reaction, and it lands as funny rather than serious because the sound keeps going just a little longer than expected.