Place it right after the boast or entrance, then follow it with a short reaction sound such as Bruh to let the joke settle before the next line. For a longer gag, open with Goofy Ahh Sounds, use this clip as the punch, and close with Emotional Damage to sell the fallout.
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This is a short talkbox clip, about 2.7 seconds long, in which a robotic, warbling voice rattles off the nonsense syllables yo boi yi bi oi ai with a wobbly, musical slur. It feels goofy and deliberately awkward, like a robot trying to hype someone up and tripping over its own words, and it lands best as a quick, silly interruption rather than a serious line.