Place this clip at the very end of a failed-joke sequence in your edit, after you build the moment up. Follow it with the sad violin sound button for the letdown, or lead into it with Nelson's Ha-Ha! sound button when the mockery needs to escalate first.
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A brief voice clip of a person flatly delivering the line 'you ain't funny' in a deadpan tone. Lasting just over four seconds, it lands with a blunt, deflating feeling that turns a failed joke into an awkward moment for everyone listening, with no laugh to soften the callout.