Place it at the end of a reveal, right after the guilty reaction appears, with a dramatic dun dun dun cueing the reveal beforehand and a vine boom hitting straight after it for the final sting.
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A sub-second shout from a single angry voice, snapping out “you bitch” with hard emphasis on the insult. It hits fast and feels confrontational, like a sudden accusation in the middle of a joke, yet the exaggeration keeps it playful rather than genuinely hostile. That mix of mock outrage and instant tension makes it work as a punchy reaction to being pranked, exposed, or unexpectedly one-upped.