Place it at the end of a confrontation beat, right after the line that crossed it, then follow with No No Wait Wait if the other person tries to backtrack, and save Oh My God Wow for the reply that comes after.
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This is a short spoken warning lasting about 3.7 seconds, delivered in a firm, raised voice that puts hard emphasis on the word stop. It lands as tense and confrontational, the kind of line that makes a room go quiet for a beat before anyone answers back.