Place this clip right after the on-screen question or mistake, before any response line lands. Follow it with the Auughhh Meme Sound Button for a second reaction, or end the sequence there if the next scene already carries the conversation forward without another effect.
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This clip is a stretched-out vocal 'eee' sound lasting about sixteen seconds, a single hesitant filler noise held far longer than a normal pause. It gives listeners an awkward, amused feeling, like watching someone stall for time while searching for an answer and coming up empty in front of everyone.